Figure 6.
An example of plasmid dependent strains. (A) The gel showing two plasmids generated from the same insertion of deletion module Tn5Del8. (B) Strains 4.6 and 4.9 are streaked on TYE medium with and without IPTG. The difference between plasmids 4.6 and 4.9 lies in the absence of glyS and glyQ in plasmid 4.6. The elimination of both plasmids may indicate that eitherglyS or glysQ, or both, are essential.
