Chromosomal Deletion Formation System Based on Tn5 Double Transposition: Use For Making Minimal Genomes and Essential Gene Analysis

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Figure 5.
Figure 5.

Microarray deletion map of deletions found in strains Δ20-1 and Δ20-4. Each dot represents the log ratio of normalized signal intensities for a particular ORF from the deletion strain to its control strain counterpart. Deletion regions were determined by consecutive signal ratios of E. coli ORFs below 1 andP-values <0.05. These hybridization results were plotted against base pair location for comparison with a NotI restriction map (Heath et al. 1992).

This Article

  1. Genome Res. 13: 644-653

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