Chromosomal Deletion Formation System Based on Tn5 Double Transposition: Use For Making Minimal Genomes and Essential Gene Analysis

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Figure 3.
Figure 3.

Deletions characterized for the lactose operon region. Genes deleted inE. coli MG1655 with the deletion transposon Tn5Del7 are shown by a gray line for each of the seven deletions in thelac operon. The insertion site was located 362,522 bp in theE. coli chromosome within the lacZ gene.

This Article

  1. Genome Res. 13: 644-653

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