(A) Expression of ten putative proteases without corresponding oligomers in the microarray set. RT-PCR was conducted to examine the transcriptional expression of the ten putative proteases using specific primers based on the prediction. Lane a in each sample represents the negative control in which RT-PCR was conducted without reverse transcriptase. Lane 1b: PF14_0281; Lane2b: PFL1635w; Lane 3b: MAL6P1.153; Lane 4b: PFL1925w; Lane 5b: PFC0950C; Lane 6b: MAL8P1.16; Lane7b: MAL6P1.88; Lane 8b: MAL8P1.128; Lane 9b: PFA0400c; Lane 10b: PFI1545c.M indicates 1 kb DNA ladder. (B) Expression of putative calpain, caspase, and SP1 genes using two pairs of primers. RT-PCR was conducted to confirm the transcriptional expression of putative calpain, metacaspase, and SP-1 genes, using 2 pairs of specific primers for each gene. Lanes “a” represent negative controls in which RT-PCR was conducted without reverse transcriptase.M indicates 1 kb DNA ladder. Lanes1b and 2b: MAL13P1.310; Lanes 3b and4b: PF13_0289; Lanes 5b and 6b: PF13_0118. See Suppl. Table 1 for the predicted size of RT-PCR products.
