Multiple alignment of the catalytic domains of the putative P. falciparum calpain (MAL13P1.310) and the representative human calpains using T-coffee program followed by manual correction. The catalytic domain region is predicted to be from amino acid residue 985 to 1453 by pfam HMM algorithm. The three conserved amino acids, C(1035), H(1371), N(1391), that are part of the active sites are highlighted with arrowheads. Graphic presentation of the alignment and the consensus sequence were obtained by the program BOXSHADE 3.21. Conserved residues are shaded with black and gray. The accession numbers of calpain protein sequences used for alignment refer to Figure2.
