Tissue-specific and imprinted expression of Slc38a4 in the mouse. (A) Northern blot hybridization of adult tissues and day-13 foetus and placenta, using a probe antisense to a portion of theSlc38a4 transcript (146–846 in AX046352). Hybridization withGapdh gives an indication of the amount of RNA loaded. (B) RT-PCR/RFLP analysis of imprinted expression ofSlc38a4 locus in inter-subspecific hybrids of C57BL/6 (B) and CAST/Ei (C). BsmI digests the C57BL/6 allele (B), as indicated in the control reactions performed on cDNA from parental strain midgestation embryos. In each case, the hybrids follow the convention maternal strain × paternal strain. There was no amplification in control reactions performed without reverse transcriptase (data not shown). M is a 100-bp molecular weight marker.
