Table 1.
Recombineering Reagents
| Strains | Genotype |
| DH10B | F− mcrAΔ(mrr–hsdRMS–mcrBC) ∅80dlacZΔM15ΔlacX74 deoR recA1 endA1 araD139 Δ(ara, leu)7649 ga/U ga/K rspL nupG |
| DY380 | DH10B [λcl857 (cro-bioA < >tet] |
| EL250 | DH10B [λcl857 (cro-bioA< >araC-P BAD flpe] |
| EL350 | DH10B [λcl857 (cro-bioA < >araC-P BAD cre] |
| Selection cassettes | |
| PL451 | FRT–PGK–EM7–NeobpA–FRT–loxP |
| PL452 | loxP–PGK–EM7–NeobpA–loxP |
| Other plasmids | |
| pSK+ | pBluescript |
| PL253 | ModifiedMC1TK |
