Figure 4.
Identification of correctly targeted ES cell clones. (A) Homologous recombination between the Evi9 cko-targeting vector and the Evi9 genomic locus. Correctly targeted ES cells (cko allele) have a 5.5-kb BglII band, in addition to an 18.1-kb wild-type band, following hybridization with the 5′ probe. These cko clones also have a 6.3-kb EcoRV-targeted band, as well as a 7.3-kb wild-type band, following hybridization with the 3′ probe. (B) Southern blot analysis of the ES cell clones. The 5′ probe was used in the left panel, and a 3′ probe was used in the right panel. (wt) Wild-type ES clones; (cko) conditional knockout ES clones.
