Subcloning a DNA fragment from a BAC into pBluescript (pSK+) by gap repair with short homology arms via recombineering. Primers that have 20 bp of homology (yellow arrows) to pBluescript (yellow circle) at their 3′ end, and 50 bp (purple or blue) of homology at their 5′ ends to one of two ends of the BAC DNA to be subcloned (light blue), are used to amplify pBluescript. The PCR-amplified, linearized pBluescript containing the two homology arms is then transformed into recombination-competent cells that carry the BAC (BAC backbone in pink color). Gap-repaired plasmids are selected by their ampicillin resistance. The black bar denotes the location of Evi9 exon 4.
