Figure 2.
Construction of the normalized cDNA library (Z2). (A) Size selection of cDNA for normalization. (Left) Total cDNA before size selection, (right) size-selected cDNA. (B) Separation of ss-cDNA and ds-cDNA on hydroxyapatite column (HA column). ss-cDNA is eluted at 100 min and ds-cDNA is eluted at 150 min. (C) Colony hybridization to examine the normalization efficiency using the β-tubulin gene as a probe. (SI) Library constructed from prenormalized cDNA; (NIII) library constructed from cDNA after three rounds of normalization.
