Deletion Phenotype of S. pombe Genes and Their Closest Homolog inS. cerevisiae
| Gene name | Class | Deletion | Deletion of closest S. cerevisiae homolog17 | |
| SPBC1271.15c | Ia | viable | viable | YOL023w/FM1 |
| SPBC1271.14 | II | viable | viable | YMR062c/ECM40 |
| SPBC1271.13 | Ia | lethal | viable | YJL063c/MRPL8 |
| SPBC1271.12 | Ib | viable | viable | YPLI45c/KES1 |
| SPBC1271.11 | Ib | viable | viable | YNL003c/PET8 |
| SPBC1271.10c | III | viable | viable | YNR055c/HOL1 |
| SPBC1271.09 | Ia | viable | viable | YCR098c/GIT1 |
| SPBC1271.08c | V | viable | — | |
| SPBC1271.07c | IV | viable | — | |
| SPBC1271.06c | V | viable | — | |
| SPBC1271.05c | III | viable | viable | YOR052c |
| SPBC1271.04c | Ia | no deletion | lethal | YHR068w/DYS1 |
| SPBC1271.03c | V | viable | — | |
| SPBC1271.02/stt3 | Ic | lethal | lethal | YGL022w/STT3 |
| SPBC1271.01c | V | viable | — | |
| SPBC106.02c | Ib | no deletion | viable | YKL086w |
| SPBC106.03 | V | viable | — | |
| SPBC106.04 | Ib | viable | viable | YML035c/AMD1 |
| SPBC106.05c | V | viable | — | |
| SPBC106.06 | Id | lethal | lethal | YDL143w/CCT4 |
| SPBC106.07c | Ic | viable | lethal | YGR147c |
| SPBC106.08c | V | viable | — | |
| SPBC106.09/cut4 | Id | lethal 2 | lethal | YNL172w/APC1 |
| SPBC106.10/pka1 | Ic | viable3 | viable | YPL203w/TPK2 |
| SPBC106.11c | IV | no deletion | — | |
| SPBC106.12c | IV | no deletion | — | |
| SPBC106.13 | Ib | no deletion | viable | YIL097w |
| SPBC106.14c | Ib | no deletion | lethal | YGR245c/SDA1 |
| SPBC106.15 | Ia | no deletion | lethal | YPL117c/IDI1 |
| SPBC106.16 | Ia | no deletion | lethal | YOL038w/PRE6 |
| SPBC106.17c | II | viable | viable | YNL277w/MET2 |
| SPBC106.18 | Ia | no deletion | lethal | YOL127w/RPL25 |
| SPBC106.19 | V | no deletion | — | |
| SPBC106.20 | Id | viable | lethal | YJL085w/EXO70 |
| SPBC582.03/cdc13 | Ib | lethal 4 | viable | YPR119w/CLB2 |
| SPBC582.04c | V | viable | — | |
| SPBC582.05c/brc1 | Ib | viable5 | viable | YHR154w/ESC4 |
| SPBC582.06c | V | viable | — | |
| SPBC582.07c | Ib | lethal | lethal | YPR108w/RPN7 |
| SPBC582.08 | Ia | viable | viable | YDR111c |
| SPBC582.09 | IV | no deletion | — | |
| SPBC582.10c | Ia | viable | viable | YBR114w/RAD16 |
| SPBC582.11c | Ib | lethal | viable | YDL116w/NUP84 |
| SPBC582.12c | Ia | viable | viable | YJR148w/BAT2 |
| SPBC428.03c | Ib | viable | viable | YAR071w/PHO11 |
| SPBC428.04 | V | viable | — | |
| SPBC428.05c | Ia | viable | viable | YOL058w/ARG1 |
| SPBC428.06c | V | viable | — | |
| SPBC428.07 | V | no PCR* | — | |
| SPBC428.08c/clr4 | Ia | viable6 | viable | YJL168c/SET2 |
| SPBHC428.09c | V | viable | — | |
| SPBC428.10 | Ic | viable | viable | YIR019c/MUC1 |
| SPBC428.11 | Ia | viable | viable | YLR303w/MET17 |
| SPBC428.12c | Ib | viable | lethal | YER165w/PAB1 |
| SPBC428.13c/mob1 | Ib | lethal 7 | lethal | YIL106w/MOB1 |
| SPBC428.14 | Ib | viable | viable | YBR042c |
| SPBC428.15 | Ia | no deletion | viable | YGR210c |
| SPBC428.16c/rhb1 | Ib | lethal 8 | viable | YCR027c/RSG1 |
| SPBC428.17c | V | viable | — | |
| SPBC428.18/cdt1 | IV | lethal 9 | — | |
| SPBC428.19c | Ib | viable | lethal | YMR093w |
| SPBC902.01c/alp6 | Ib | lethal 10 | lethal | YNL126w/SPC98 |
| SPBC902.02c | Ia | viable | viable | YMR078c/CTF18 |
| SPBC902.03 | III | viable | viable | YAL009w/SPO7 |
| SPBC902.04 | IV | viable | — | |
| SPBC902.05c/glu2 | Ia | viable11 | viable | YOR136w/IDH2 |
| SPBC902.06 | V | viable | — | |
| SPBC1685.01/pmp1 | Ib | viable12 | viable | YIL113w |
| SPBC1685.02c | Ib | viable | viable | YOR369c/RPS12 |
| SPBC1685.03 | Ia | lethal | lethal | YIR022w/SEC11 |
| SPBC1685.04 | V | viable | — | |
| SPBC1685.05 | II | viable | viable | YNL123w |
| SPBC1685.06 | Ib | viable | viable | YNL299w/TRF5 |
| SPBC1685.07c | Ib | viable | viable | YBL089w |
| SPBC1685.08 | Ib | viable | viable | YPL181w |
| SPBC1685.09 | Ia | viable | viable | YLR388w/RPS29A |
| SPBC1685.10 | Ia | no deletion | viable | YHR021c/RPS27B |
| SPBC1685.11 | V | viable | — | |
| SPBC1685.12c | non- | coding ? | ||
| SPBC1685.13 | III | viable | viable | YPR149w/NCE102 |
| SPBC1685.14c | Ib | viable | viable | YNL212w/VID27 |
| SPBC649.01c/klp6 | Ib | viable13 | viable | YGL216w/KIP3 |
| SPBC649.02 | Ia | viable | viable | YNL302c/RPS19B |
| SPBC649.03 | Id | viable | viable | YMR201c/RAD14 |
| SPBC649.04/uvi15 | V | viable14 | — | |
| SPBC649.05/cut12 | V | lethal 15 | — | |
| SPBC649.06 | Ia | viable | viable | YGR173w |
| SPBC354.02c/sec61 | Ia | lethal 16 | lethal | YLR378c/SEC61 |
| SPBC354.03 | Ib | no PCR* | lethal | YBR198c/TAF90 |
| SPBC354.04 | V | no PCR* | — | |
| SPBC354.05c | Id | no deletion | viable | YOR032c/HMS1 |
| SPBC354.06 | Ia | no deletion | viable | YPL013c |
| SPBC354.07c | Ib | viable | viable | YPL145c/KES1 |
| SPBC354.08c | Ib | viable | viable | YMR266w |
| SPBC354.09c | Ib | viable | viable | YPL176c |
| SPBC354.10 | Ia | viable | viable | YIR019c/MUC1 |
| SPBC354.11c | V | viable | — | |
| SPBC354.12 | Ia | no deletion | viable | YJL052w/TDH1 |
| SPBC354.13 | Id | no deletion | viable | YDR389w/SAC7 |
| SPBC354.14c | Ib | viable | viable | YEL013w/VAC8 |
| SPBC354.15 | IV | viable | — | |
-
Fission yeast genes from chromosome II were deleted in a diploid strain using targeted PCR-based gene deletion procedure (Bähler et al., 1998). Diploids were sportulated and the spore viability was assessed on rich glucose medium. Deletion phenotype of the closest homolog in S. cerevisiae (if any) is given.
-
↵ Yoshida et al. (1999), 2 Yamashita et al. (1996),3 Fernandez et al. (1997), 4 Booher and Beach (1988),5 Verkade et al. (1999), 6 Bannister et al. (2001)and Nakayama et al. (2001), 7 Hou et al. (2000),8 Mach et al. (2000), 9 Hofmann and Beach (1994),10 Vardy and Toda (2000), 11 Barel and MacDonald (1993), 12 Sugiura et al. (1998) 13our study andWest et al. (2002), 14 Lee et al. (1995), 15 Bridge et al. (1998), 16 Broughton et al. (1997), 17YPD (see Garrels, 2002) and EUROSCARF (http://www.uni-frankfurt.de/fb15/mikro/euroscarf/). *No amplification of the deletion cassette.
