Table 2.
Ranges of TSS Discrepancies and Promoter Activities of the 152 Experimental DNA Fragments
| TSS difference (bp) | Experimental promoters | Avg. strength of positives | ||||
| number | percent | negative | positive | % positive | ||
| 0 | 58 | 38.2 | 5 | 53 | 91.4 | 305 |
| 1–100 | 47 | 30.9 | 1 | 46 | 97.6 | 386 |
| 100–300 | 12 | 7.9 | 1 | 11 | 91.7 | 297 |
| 300–500 | 7 | 4.6 | 0 | 7 | 100.0 | 174 |
| 500–1000 | 7 | 4.6 | 0 | 7 | 100.0 | 131 |
| >1000 | 21 | 13.8 | 7 | 14 | 66.7 | 30 |
| Total | 152 | 100.0 | 14 | 138 | 90.8 | 289 |
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TSS difference is the discrepancy in length (base pairs) between the putative TSS predicted by MGC clones and the TSS predicted by the longest transcript available from RefSeq or DBTSS. When the TSS difference is 0, the MGC clone is the longest available. Due to the size of the fragments we are testing (∼500 bp), those TSS differences that are less than 500 bp contain sequences just upstream of the TSS of the longest transcript described above.
