Systematic Discovery of New Genes in the Saccharomyces cerevisiae Genome

Table 1.

List of S. cerevisiae smORFs for Which a Transcript was Detected

smorf Original name Chromososme Coordinates Size (aa) CAI RT-PCR product (bp) SGD annotation Notes
3 SR13 chr1 124491–124306 62 0.243 39
13 chr2 18437–18177 87 0.181 99
18a smORF2 chr2 89973–90221 82 0.154 205 YBL071W-A
19c SR12 chr2 91789–92025 79 0.241 200 YBL069W embedded ORF
24 SR11 chr2 209370–209609 79 0.102 142
44 SR10 chr2 382978–382817 54 0.134 93
46 chr2 391575–391309 89 0.091 72
53 SR9 chr2 614131–613982 50 0.125 115
54 SR8 chr2 622941–623105 55 0.082 113
57a b SR7 chr2 684935–685219 94 0.105 170 YBR233W-A
68 chr3 24325–24032 98 0.149 99 YCL057C-A removed by S GD
82a SR6 chr3 113722–114018 98 0.102 247 YCL001W-B
93 SR5 chr3 316181–315990 63 0.121 161
98 SR4 chr4 229171–229431 87 0.175 38
101 SR3 chr4 410052–409804 83 0.151 68
104 chr4 410052–409804 83 0.108 77
109a b chr4 603805–603587 73 0.119 31 YDR079C-A
118 SR2 chr4 794716–794567 50 0.164 124/98e
121 SR1 chr4 829143–829346 67 0.160 155
122 chr4 830646–830389 86 0.149 31
123 chr4 848063–848215 51 0.067 64
127 chr4 955124–955324 67 0.066 99
139a smORF8 chr4 1233506–1233267 79 0.161 227 YDR379C-A
144 chr4 1283788–1284033 82 0.153 34
154 chr9 96725–96522 78 0.135 67
167 chr5 59549–59740 77 0.099 41
171 chr5 117380–117183 66 0.216 87
189 chr5 318642–318806 86 0.065 97
201 chr6 48734–48925 64 0.173 63
217 chr7 93078–93308 77 0.078 99
226 chr7 418882–418700 61 0.097 40
247 chr7 733407–733622 72 0.118 31
250 chr7 974773–974573 67 0.091 92
274 chr8 280232–280393 54 0.095 79
283 chr8 453556–453705 50 0.084 75
286 chr8 467058–466954 55 0.102 74
288 chr8 466928–467110 61 0.079 62
298 chr10 181408–181250 53 0.158 99
301a b chr10 316419–316676 86 0.106 99 YJL062W-A
303a chr10 411124–410927 66 0.183 94 YJL012C-A
313 chr10 637622–637861 80 0.081 99
318 chr10 717273–717323 74 0.132 42
324 chr11 98609–98397 71 0.186 25
337 chr11 447674–447432 81 0.156 27
352 chr12 136344–136520 59 0.197 85
363 chr12 455434–455637 68 0.105 34
382 chr12 455402–455566 55 0.078 99
392 chr12 454697–455071 125 0.170 98
398 chr12 454393–454557 55 0.059 99
421 chr12 462672–462523 50 0.045 99
439 chr12 452439–452624 62 0.086 99
483 chr12 490407–490595 63 0.131 65
494d SR15 chr12 468959–468831 44 0.052 100
472611–472483
482191–482063
485843–485715
499 chr12 582236–582006 77 0.140 37
505 chr12 708338–708168 57 0.105 45
509 SR17 chr12 760642–760355 96 0.102 190/82e
511 SR16 chr12 815810–815983 58 0.105 125/61e
514a SR18 chr12 853460–853717 85 0.282 230 YLR363W-A
519 chr12 950468–950262 69 0.135 39
526 chr13 167781–167623 53 0.129 99
530 SR25 chr13 337312–337602 97 0.082 160/58e
532 chr13 550910–551134 75 0.121 94
540 chr13 733267–733455 63 0.158 64
543 chr13 804455–804691 79 0.101 80
544 chr13 887925–887731 65 0.094 59
556a SR22 chr14 330326–330544 73 0.130 170/37e YNL162W-A
561 SR21 chr14 381388–381242 48 0.102 90
564 chr15 4130–4312 61 0.121 55
570 smORF31 chr14 586816–386598 72 0.124 216
577 chr15 4130–4312 61 0.153 75
580 chr15 9179–9352 58 0.222 77
590 chr15 355651–355857 78 0.135 29
591 chr15 371684–371956 63 0.139 99
598 chr15 461792–462040 59 0.054 99
601 chr15 467391–467624 70 0.103 67
625 chr15 907927–907718 70 0.085 97
626 chr15 939340–939549 70 0.111 67
631 chr15 1045189–1045344 52 0.117 78
632 chr15 1058416–1058583 56 0.154 73
640 chr16 75702–75989 96 0.158 99
643 chr16 188512–188306 69 0.133 84
655 chr16 480177–480368 64 0.151 30
667 chr16 744172–744384 71 0.087 81
672 chr16 883234–883452 73 0.129 95

  • This table indicates the chromosomal location based on SGD nucleotide coordinates. smORFs marked with an (a) correspond to those identified by Blandin et al. (2000). smORFs marked with a (b) sign correspond to those identified by Cliften et al. (2001). smorf19 (c) was originally located downstream of YBL069W (AST1), which was recently extended (Saccharomyces Genome Database, 2001.http://www.genome-www.standford.edu/Saccharomyces). smorf494 (d) is found four times in chromosome 12, downstream of theASP3 gene in a 3.6-kb repeat (Johnston et al. 1997). For smORFs marked with (e), RT-PCR was carried with two sets of primers, one set shown in Fig. 2D and a second in Fig. 2E.

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  1. Genome Res. 13: 264-271

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