Systematic Discovery of New Genes in the Saccharomyces cerevisiae Genome

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Figure 4.
Figure 4.

smORF2 is expressed in yeast. A triple HA tag was fused to the C-terminal end of smORF2 using PCR, and the wild-type smORF2 gene was replaced by the tagged smORF2 gene by allele replacement into the chromosome. Soluble extracts were prepared and analyzed in a Western blot probed with monoclonal antibodies that recognize the HA epitope. Extracts from wild-type cells (lane 2) and extracts from two separate isolates carrying the HA-tagged smORF2 (lanes3,4).

This Article

  1. Genome Res. 13: 264-271

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