Integration of Ty1-neo into SNR6- andSUP2-containing target plasmids. (A) U6mgrepresents the marked SNR6 gene, and (B)sup2+b is the marked SUP2 gene. The term genomic signifies that the sequence is the native sequence flanking theSNR6 or SUP2 loci, respectively, whereas2μ and HIS3 are vector sequences. Ty1-neoinsertions into the target plasmids are depicted as small black arrowheads. Arrowheads pointing downward represent Ty1-neoinsertions in the same transcriptional direction as the target gene, whereas those pointing upward are transcribed divergently from the target gene. The distance in base pairs from the major pol III transcription start sites (+1) is indicated (Kinsey and Sandmeyer 1991;Chalker and Sandmeyer 1993). The lowercase letters indicate the individual Ty1-neo insertion constructs that were used for expression studies.The Ty1-neo integration positions are −273, −147, −90, +318, −608, −289, −96, and −83 for a–h and −166, −105, −179, and −103 for t, u, w, and x, respectively.
