Summary of the Chicken Physical Map
|
Number of clones fingerprinted |
66,048 |
| Number of clones with successful fingerprints | 58,079 |
| Number of clones used for map contig assembly | 57,091 |
| Number of singletons | 6,830 |
| Number of contigs | 2,331 |
| >200 clones | 1 |
| 101—200 clones | 29 |
| 51—100 clones | 206 |
| 26—50 clones | 476 |
| 10—25 clones | 603 |
| 3—9 clones | 812 |
| 2 clones | 204 |
| Physical length of the contigs
|
1,510 Mba
|
-
↵a Each fingerprint band was estimated to represent an average of 4.44 kb. It was estimated by the average insert size of the BAC clones (158 kb; Table 1; Lee et al. 2003) divided by the average number of valid bands per clone (35.6 bands per clone = 2,066,401 bands in total/58,079 clones) used for the map contig assembly. The average insert size of the clones was estimated from the NotI digestion of 944 clones selected randomly from the three source BAC libraries, followed by pulsed-field gel electrophoresis (Lee et al. 2003). Unlike fingerprinting on agarose gels, the sample DNAs were double digested with HindIII and HaeIII, but only end-labeled at the HindIII sites, so that the bands without HindIII sites were not shown on the autoradiographs by fingerprinting on polyacrylamide gels
