Analysis and Functional Annotation of an Expressed Sequence Tag Collection for Tropical Crop Sugarcane

Table 3.

Sequence Assembly Results


Total sequences analyzed

237,954
Number of contigs 26,803
Number of singletons 16,338
Number of sugarcane assembled sequences (SAS) 43,141
Number of assembled sequences matching known genes 27,833
Number of clones with full-length inserts
14,409
  • High-quality ESTs were assembled using CAP3. A BLASTX match cutoff of ≤10—5 was used to assign similarity. Clones were considered as having a putative full-length insert when their sequences started within the first 15 amino acids of their best BLASTX match with an E = value ≤10—40.

This Article

  1. Genome Res. 13: 2725-2735

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