Table 1.
Summary of Amplicons Screened and Mutations Found
|
Gene |
Amplicon |
Screening |
No. of mutations |
|
||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Name |
Cds (bp) |
Name |
Length/cdsc(bp) |
Success (%) |
Screened (Mb) |
Total |
Recovered |
Nonsense |
Splice |
Missense |
Silent |
Noncoding |
Mutation frequency (bp/mutation) |
|||||||||
| gene1 | 690 | A | 569/264 | 88 | 2.3 | 16 | 6 | — | — | — | 1 | 5 | 384,553 | |||||||||
| gene2 | 1434 | A | 777/381 | 74 | 2.6 | 28 | 15 | — | 1 | 6 | 2 | 6 | 176,634 | |||||||||
| gene3 | 1821 | A | 639/327 | 80 | 2.4 | 22 | 18 | 1 | 2 | 7 | 3 | 5 | 130,867 | |||||||||
| gene4 | 4860a | A | 911/879 | 81 | 3.4 | 21 | 14 | 1 | — | 8 | 5 | — | 242,878 | |||||||||
| gene5 | 1239 | A | 812/327 | 76 | 2.8 | 24 | 18 | 2 | 1 | 2 | 3 | 10 | 157,983 | |||||||||
| gene6 | 951 | A | 541/436 | 33d | 0.8 | 6 | 6 | 1 | — | 2 | 3 | — | 137,111 | |||||||||
| gene7 | 2271b | A | 590/264 | 68 | 1.8 | 24 | 6 | — | 1 | 2 | 1 | 2 | 308,122 | |||||||||
| gene8 | 3240b | A | 442/270 | 79 | 1.6 | 14 | 6 | — | 1 | 2 | 2 | 1 | 268,170 | |||||||||
| gene9 | 1122 | A | 841/279 | 78 | 3.0 | 34 | 20 | — | — | 7 | 2 | 11 | 151,138 | |||||||||
| B | 448/230 | 81 | 1.7 | 9 | 7 | — | 1 | 2 | 2 | 2 | 238,879 | |||||||||||
| gene10 | 1410 | B | 664/395 | 64 | 2.0 | 8 | 4 | 1 | — | 3 | — | — | 489,554 | |||||||||
| gene11 | 4110 | A | 720/720 | 87 | 2.9 | 27 | 11 | — | — | 6 | 5 | — | 262,405 | |||||||||
| gene12 | 480 | A | 496/469 | 87 | 2.0 | 13 | 6 | — | — | 3 | 3 | — | 331,407 | |||||||||
| gene13 | 2151 | A | 816/443 | 75 | 2.8 | 17 | 9 | 1 | — | 4 | 1 | 3 | 313,344 | |||||||||
| gene14 | 8532b | A | 953/953 | 83 | 3.6 | 28 | 16 | — | — | 14 | 2 | — | 227,805 | |||||||||
| B | 873/873 | 80 | 3.2 | 22 | 13 | 1 | — | 7 | 5 | — | 247,556 | |||||||||||
| C | 813/813 | 91 | 3.4 | 20 | 10 | — | — | 5 | 5 | — | 340,914 | |||||||||||
| G | 921/881 | 87 | 3.7 | 22 | 11 | 1 | — | 6 | 4 | — | 335,659 | |||||||||||
| gene15 | 6912b | A | 955/955 | 89 | 4.4 | 25 | 18 | 2 | — | 12 | 4 | — | 244,480 | |||||||||
| gene16 | 6524 | A | 798/775 | 90 | 3.3 | 26 | 17 | 2 | — | 9 | 6 | — | 194,674 | |||||||||
| C | 752/418 | 93 | 3.2 | 32 | 16 | 1 | — | 8 | 2 | 5 | 201,416 | |||||||||||
| E | 736/526 | 81 | 2.7 | 20 | 8 | — | — | 4 | 2 | 2 | 343,388 | |||||||||||
| Average | 2984 | 730/540 | 79 | 2.7 | 20.8 | 11.6 | 0.6 | 0.3 | 5.4 | 2.9 | 2.4 | 234,625 | ||||||||||
| Total
|
47,747
|
|
16,067/11,878
|
|
59.8
|
458
|
255
|
14
|
7
|
119
|
63
|
52
|
|
|||||||||
-
↵a Length of complete coding sequence/cds is based on Drosophila melanogaster homolog
-
↵b Length of complete cds is based on Homo sapiens ortholog
-
↵c Length of nested PCR amplicon, excluding primer sequences; length of cds in amplicon
-
↵d Library not completely screened for this amplicon; false positives not counted
