Figure 2
Representative example of a gel used to screen 384 fish for mutations in the dicer gene. Each of the 96 lanes of the polyacrylamide gel contains fourfold-pooled CEL-1-digested PCR products of ∼830 bp. The IR Dye 700 and IR Dye 800 channels are shown at left and right, respectively. Arrowheads indicate nine SNPs present in the founder fish. SNP no. 5 is only visible in one channel, and therefore, indicated by a gray arrowhead. Arrows indicate the three potential mutations on this gel (all rank 1).
