A Strategy for Constructing Large Protein Interaction Maps Using the Yeast Two-Hybrid System:Regulated Expression Arrays and Two-Phase Mating

Table 1.

Construction of 3D Pools

Plane
No. of pools
No. of clones per pool
Example of pools
XY (plate) 24 192 Plate #1 plus #25
XZ (row) 24 192 Row A from Plate #1 to #16
YZ (column)
24
192
Column 1 from Plate #1 to #24

  • Pools are constructed from a stack of 48 plates, each with 96 wells as illustrated in Figure 5.

This Article

  1. Published in Advance January 1, 2003, doi: 10.1101/gr.1134603 Genome Res. 13: 2691-2699

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