A Strategy for Constructing Large Protein Interaction Maps Using the Yeast Two-Hybrid System:Regulated Expression Arrays and Two-Phase Mating

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Figure 5
Figure 5

A 3D pooling scheme. A stack of 96-well plates represents a three-dimensional space defined by X, Y, and Z axes. AD pools are constructed from plates, which are in the XY plane, rows in the XZ plane, and columns in the YZ plane. If a BD interacts with three orthogonal AD pools, one from each plane, the interacting AD is indicated by the only common AD among all three pools. Pools can be constructed to contain an equal number of clones as shown in Table 1.

This Article

  1. Genome Res. 13: 2691-2699

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