Figure 2
McrPCR. Equal samples of genomic DNA are either pretreated with CpG methylase (Sss I methylase), or not, and both samples are digested in a time course with McrBC (only time zero and complete digestion are shown). PCR is then performed using specific primers (arrows). If the target sequence (gray box) is methylated in the genome, there will be a decrease in the amount of PCR product following McrBC digestion with or without Sss I methylase pretreatment. If the target is not methylated, a decrease in PCR yield will only be evident following Sss I methylase pretreatment.
