Table 1.

Features of Genes That Differ Between Those That Exhibit or Lack an Observed RNAi Phenotype

	Occurrence in loci with RNAi phenotypes^a
	Higher		Lower
Amino Acid Characteristics
Number of Residues^b^**	^****
Total Molecular Weight^d	^****
Average Residue Weight			^****
Total Cost^d	^****
Cost per Residue			^****
Essential Residues (%)			^****
Total Protein Charge			^****
Charged Residues (%)	^****
Isoelectric Point			^****
Tiny Residues (%)		ns
Small Residues (%)	^***
Aliphatic Residues (%)			^****
Aromatic Residues (%)			^****
Polar Residues (%)	^****
Basic Residues (%)	^****
Acidic Residues (%)	^****
Amino Acid Composition (%)
Alanine	^****
Arginine	^****
Asparagine			^****
Aspartate	^****
Cysteine			^****
Glutamic Acid	^****
Glutamine	^****
Glycine	^****
Histidine		ns
Isoleucine			^****
Leucine			^****
Lysine	^****
Methionine		ns
Phenylalanine			^****
Proline		ns
Serine			^****
Threonine			^****
Tryptophan			^****
Tyrosine			^****
Valine	^**
Expression Levels
Average across development^b^****	^****
Sequence Characteristics
F_op	^****
G+C Content	^****
Fraction Coding^b^****	^****
K_A/K_S^d			^****
K_S^b^****^c			^****
K_A^b^****			^****

↵a Significance levels from Wilcoxon rank sum test indicated by ^****P < 0.0001, ^***P < 0.001 ^**P < 0.01, ^*P < 0.05, (ns) no significant difference (P > 0.05)
↵b Significance in logistic multiple regression analysis with asterisk P-value cutoffs as in ^a
↵c K_S is significantly higher among loci with RNAi phenotypes in the multivariate analysis
↵d Not included in multivariate analyses

Molecular Correlates of Genes Exhibiting RNAi Phenotypes in Caenorhabditis elegans