Ability of combinations of different species' sequences to detect MCSs. Sequences from every combination of the 11 non-human species were analyzed by the binomial-based method, and the subset of each possible number of species (from 1 to 10, in addition to human) yielding the highest sensitivity at 75% specificity was identified. Note that the ranking of the subsets remains essentially the same for a wide range of specificity thresholds. (A) The resulting relationship between sensitivity and specificity is shown for each subset (see Fig. 7A for details). (B) Detection of reference MCS bases (see Fig. 7B for details), shown for each best-performing subset of species using data obtained with a specificity of 75% (horizontal grey line in A). Note that the far-left bar represents the entire set of reference MCS bases (see Fig. 7B). The underlying data associated with these analyses are available at http://www.nisc.nih.gov/data.
