Figure 4
Correlation between siRNA screening results and suppression of endogenous Lamin A/C. (A) The location of the siRNAs against the Lamin A/C gene. (B) HeLa cells were transfected with a constant amount of EGFP-Lamin A/C, pDsRed2-N1, and specific Lamin A/C siRNA and nonspecific siRNA. Normalized relative amounts of EGFP and RFP for each siRNA and % reduction in GFP fluorescence for each siRNA are shown. (C) Western blot analysis using α-Lamin A/C antibody.
