Figure 2
Quantitation of siRNA-mediated target gene silencing. (A) C2C12 cells were transfected with a constant amount of EGFP-Rluc (expressing EGFP-Renilla luciferase fusion protein), pGL3-Control (expressing firefly luciferase as internal control), and increasing concentrations of EGFP-specific siRNA (upper panel) and nonspecific siRNA (lower panel). At 24 h posttransfection cells, were imaged for EGFP expression. (B) Normalized relative amount of Renilla and firefly luciferase (n=3) as a function of treatment with nonspecific siRNA (▪) or EGFP-specific siRNA (▴) were plotted. Cotransfection without siRNA served as a control. A scrambled siRNA served as a nonspecific control.
