Physical Maps for Genome Analysis of Serotype A and D Strains of the Fungal Pathogen Cryptococcus neoformans

Table 1.

Summary of the Hybridization of Selected Markers to BAC Clones of H99 and JEC21

Probe origin Hybridization format Strain Probes successful/attempted Total positive BAC clones Average BAC clones/probe Markers mapped
Genes/ESTs Pooled JEC21 79/96 1175 14.9 56
H99 88/96 1307 14.9 76
BAC ends Individual JEC21 14/15 351 23.4 13
H99 15/15 428 28.5 15
Karyotype markers Individual JEC21 14/14 505 36.1 13
H99 11/14 280 20.0 11
  • Probes were hybridized to a total of 9216 JEC21 and 6528 H99 BAC clones. Pooled probes were used in the Overgo format described by Ross et al. (1999). Unsuccessful probes failed to hybridize or gave ambiguous results.

This Article

  1. Genome Res. 12: 1445-1453

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