Table 1.

Products from cleavage of IL 12 polymerase chain reaction product with uracil-N-glycosylase.

Expected fragments		Expected mass (da)		Allele in which fragment is seen		Observed mass (Da)
Expected fragments		Expected mass (da)		Allele in which fragment is seen		+/+	+/−	−/−
GCC	1005.6	+/−	1007.38	1004.93	1006.13
CAA	1013.6	−	N/O	1012.41	1014.22
CGA	1029.6	+	1028.69	1028.57	N/O
AAA	1037.6	+/−	1035.85	1036.69	1037.89
AGA	1053.6	−	N/O	1053.69	1055.88
GGG	1085.6	+/−	1085.97	1084.61	1088.01
AGAC	1342.8	+/−	1343.55	1340.09	1343.63
AGGA	1382.8	+/−	1379.17	1382.13	1383.46
CACAA	1616.0	+/−	1615.27	1615.74	1617.10
CAAGC	1632.0	+	1629.59	1632.72	N/O
AAACA	1640.0	+/−	1636.75	1639.14	1640.87
AAAGA	1680.0	+/−	1679.71	1678.97	1680.74
AAAACG	1969.2	+/−	1965.75	1969.36	1971.46
GAAACAA	2282.4	+/−	2284.10	2285.29	2282.65
AAACGAAA	2595.6	+/−	2591.63	2594.18	2597.34
GCAAAAAAA	2908.8	+/−	2906.31	2912.22	2908.53
AGAAACGACA	3214.0	+/−	3210.34	3211.75	3216.05

Mutation Detection Using Mass Spectrometric Separation of Tiny Oligonucleotide Fragments