Mutation Detection Using Mass Spectrometric Separation of Tiny Oligonucleotide Fragments

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

Figure 3.
Figure 3.

Partial spectra of uracil-N-glycosylase (UNG) digest of the forward strand of polymerase chain reaction product amplified using mouse genomic DNA from strains SJL (top) and C57BL/6 (bottom) showing digest fragment spanning single nucleotide polymorphism site with an ∼16 Da difference in fragment size owing to the difference in molecular weight between the bases “A” 313.21 Da and “G” 329.21 Da.

This Article

  1. Published in Advance August 22, 2002, doi: 10.1101/gr.157802 Genome Res. 12: 1428-1433

Preprint Server



Current Issue

  1. March 2026, 36 (3)
  1. Current Issue
  1. Alert me to new issues of Genome Research