Figure 5.
Melting curves of three DNA samples. Negative derivatives of DASH melting curves for PCR products for homozygous match (solid line), heterozygous (filled circles), and homozygous mismatched (hollow circles). (A) Secondary structure (no probe) signals are compared with (B) probe/target duplex signals created by Sybr Green I and (C) iFRET detection schemes.
