Table 5.
B3501 Tags More Highly Expressed at 37°C
| SAGE tag | B3501 37 | B3501 25 | 25 normalized | FOLD difference | Preliminary gene designation | E-value | Accession no.of BLASTx | ||||||||
| Genome BLASTx | EST BLASTx | ||||||||||||||
| atatgaaaga | 55 | 5 | 1 | 55.0 | NO HITS | ||||||||||
| aggaagagaa | 125 | 186 | 44 | 2.8 | Hypothetical protein | 4.00E-22 | Agaris bisporus AJ271701 | ||||||||
| acgtaccttt | 41 | 21 | 5 | 8.2 | NO HITS | ||||||||||
| cgacagaccg | 207 | 529 | 124 | 1.7 | Translation elongation factor 1α | 0.00E + 00 | 0.00E + 00 | Cryptococcus neoformans U81804 | |||||||
| ggaatttgct | 24 | 17 | 4 | 6.0 | NO HITS | ||||||||||
| tagacagact | 15 | 6 | 1 | 15.0 | Carboxypeptidase D | 1.00E-121 | P. janthinellum AAB35195.1 | ||||||||
| accgacgtga | 22 | 19 | 4 | 5.5 | Aconitate hydratase | 1.00E-165 | Piromyces sp Y16747 | ||||||||
| cggaaaaaac | 7 | 0 | 0 | unique to 37 | Hypothetical protein | 1.00E-11 | Arabidopsis thaliana AC002294 | ||||||||
| cctgttctcg | 36 | 51 | 12 | 3.0 | NO HITS | ||||||||||
| gccgcttctg | 13 | 6 | 1 | 13.0 | Ubiquinol-cytochrome C reductase iron-sulfur | 1.00E-84 | Neurospora crassa X02472 | ||||||||
| aaccagcggt | 8 | 1 | 0 | 34.1 | Salicylate hydroxylase | 3.00E-12 | Streptomyces coelicolor AL035707 | ||||||||
| aagacatcgt | 9 | 2 | 0 | 19.2 | NO HITS | ||||||||||
| attttagaaa | 9 | 2 | 0 | 19.2 | NADH-ubiquinone oxidoreductase subunit | 6.00E-07 | N. crassa X60829 | ||||||||
| gtccataagg | 13 | 7 | 2 | 6.5 | NO HITS | ||||||||||
| taactcgcat | 6 | 0 | 0 | unique to 37 | NO HITS | ||||||||||
| tctaagtata | 6 | 0 | 0 | unique to 37 | NO HITS | ||||||||||
| aacgtctgcc | 45 | 80 | 19 | 2.4 | NO HITS | ||||||||||
| cgcgcgatgc | 16 | 14 | 3 | 5.3 | NO HITS | ||||||||||
| gcattggcgt | 39 | 70 | 16 | 2.4 | ER chaperone BiP | 0.00E + 00 | 5.00E-20 | Aspergillus oryzae AB030231 | |||||||
| catctggatg | 5 | 0 | 0 | unique to 37 | NO HITS | ||||||||||
| tgttatcggt | 16 | 15 | 4 | 4.0 | Heat shock protein 80 | 1.00E-135 | N. crassa AL513463 | ||||||||
| gcattttggg | 18 | 20 | 5 | 3.6 | Ubiquinol-cytochrome C | 1.00E-34 | N. crassa Y08841 | ||||||||
| aaccgcacca | 29 | 46 | 11 | 2.6 | Reductase core protein peripheral benzodiazepine receptor—human | 6.00E-16 | Homo sapiensJE0149 | ||||||||
| tgtagtatct | 13 | 11 | 3 | 4.3 | NO HITS | ||||||||||
| tcgagtttca | 11 | 8 | 2 | 5.5 | NO HITS | ||||||||||
| attgagatgg | 44 | 91 | 21 | 2.1 | NO HITS | ||||||||||
| ctaggttatg | 4 | 0 | 0 | unique to 37 | 3′ to 5′ DNA/RNA helicase | 1.00E-165 | Schizosaccharomyces pombe AL590902 | ||||||||
| ccgcctgccg | 4 | 0 | 0 | unique to 37 | NO HITS | ||||||||||
| gctgcaagcg | 4 | 0 | 0 | unique to 37 | Hypothetical protein | 2.00E-43 | N. crassa AL513463 | ||||||||
| ttcgcggtag | 4 | 0 | 0 | unique to 37 | NO HITS | ||||||||||
| gtgatggtgg | 4 | 0 | 0 | unique to 37 | NO HITS | ||||||||||
| ccctacgaga | 4 | 0 | 0 | unique to 37 | NO HITS | ||||||||||
| atcgcgatgt | 4 | 0 | 0 | unique to 37 | Putative protein | 4.00E-05 | Mus musculusNM_025872 | ||||||||
| gggagccata | 4 | 0 | 0 | unique to 37 | NO HITS | ||||||||||
| atcctttgtc | 4 | 0 | 0 | unique to 37 | NO HITS | ||||||||||
| actcaaccgt | 10 | 7 | 2 | 5.0 | NO HITS | ||||||||||
| catagttggt | 27 | 47 | 11 | 2.5 | Heat shock protein 70 family | 0.00E + 00 | Malassezia sympodialisAJ428052 | ||||||||
| ctcaagaagg | 17 | 22 | 5 | 3.4 | Subtilisin-like serine protease | 4.00E-91 | Penicillium citrinumAF098517 | ||||||||
| tcagaaccgt | 6 | 2 | 0 | 12.8 | NO HITS | ||||||||||
| cagaacaaag | 5 | 1 | 0 | 21.3 | protein with similarity to GAPDH | 3.00E-28 | Mesorhizobium lotiAP003004 | ||||||||
| tatggctgga | 5 | 1 | 0 | 21.3 | NO HITS | ||||||||||
| gaagtccgga | 5 | 1 | 0 | 21.3 | NO HITS | ||||||||||
| tacactgtcg | 12 | 12 | 3 | 4.0 | NO HITS | ||||||||||
| gtttatggaa | 11 | 11 | 3 | 3.7 | Heat shock protein 60 | 0.00E + 00 | Coccidioides immitisU81786 | ||||||||
| aacgtaaagc | 7 | 4 | 1 | 7.0 | NO HITS | ||||||||||
| gtgtggggca | 7 | 4 | 1 | 7.0 | NO HITS | ||||||||||
| caacgtagaa | 7 | 4 | 1 | 7.0 | NO HITS | ||||||||||
| tcatcaccat | 7 | 4 | 1 | 7.0 | Translation initiation factor 3 | 6.00E-15 | Myxococcus xanthusAF261103 | ||||||||
| aactcgtgaa | 15 | 20 | 5 | 3.0 | Hypothetical protein | 2.00E-22 | 5.00E-05 | A. bisporus AJ271701 | |||||||
| gcggtgggat | 14 | 18 | 4 | 3.5 | NO HITS | ||||||||||
-
3′ or 5′ denotes that a second serial analysis of gene expression (SAGE) tag was found and that the tag is either the 3′ most tag or more 5′. Tags in this table are differentially expressed with a statistic significance of P < 0.05.
-
↵Tag did not have a corresponding contig at Stanford and did not have a corresponding expressed sequence tag (EST) at Oklahoma.
-
↵No significant BLAST hit results for the genomic or EST sequence associated with the SAGE tag.
