Temperature-Regulated Transcription in the Pathogenic Fungus Cryptococcus neoformans

Table 3B.

Top 50 Tags Expressed at 37°C for Strain B3501

SAGE tag Frequency (15,363 total) Percentage Preliminary gene designation E-value of top BLASTx result Accession no. of BLASTx
cgacagaccg 207 1.35% Translation elongation factor 1 0.0/0.0 C. neoformans U81804
aggaagagaa 125 0.81% Hypothetical protein (Agaricus bisporus) 2.00E-22/5.00E-05 A. bisporus AJ271701
aaaaaaaaaa 119 0.77% NO HITS
gcgttacttg 85 0.55% Zinc transporter 2.00E-27 S. cerevisiae Z72777
ctccgccgag 85 0.55% Pyruvate decarboxylase 1.00E-72/3.00E-40 Pichia stipitis U75310
gtcgtagagt 82 0.53% Enolase 1E-131 S. cerevisiae J01322
gtcggtggta 76 0.49% ATP synthase—β chain 0.0/3.00E-43 K. lactis U37764
ccagaagttg 68 0.44% Mitochondrial thioredoxin 2.00E-39/2.00E-72 S. cerevisiae X59720
aatgactttt 68 0.44% NO HITS
atatgacata 67 0.44% Glycine dehydrogenase 0 S. pombe Z54308
cgagtcgtat 62 0.40% Iron permease 2E-23 S. pombe Z67998
catttacata 57 0.37% NO HITS
ttcggcaagg-5′ 57 0.37% ADP, ATP carrier protein 1E-115/1.00E-132 G. hirsutum AF006489
gaacgatgct 56 0.36% NO HITS
atatgaaaga 55 0.36% NO HITS
cgcggaaagg 54 0.35% NO HITS
gtattgaccc 52 0.34% Phosphoketolase 1.00E-165/1.00E-106 Lactococcus lactis AE006381
tctttgatgt-3′ 49 0.32% ADP, ATP carrier protein 1.00E-110/2.00E-72 Gossypium hirsutum AF006489
aacgtctgcc 45 0.29% NO HITS
attgagatgg 44 0.29% NO HITS
atttccgccg 43 0.28% Serine-threonine protein kinase 7E-65 M. musculus U48737
actaccttct 42 0.27% Ribosomal protein RPP1 1.00E-13 C. elegans AF003139
acgtaccttt 41 0.27% NO HITS
cacaatcctt 41 0.27% Ubiquitin/ribosomal protein RPS27A fusion protein 6.00E-36/7.00E-40 N. crassa U01220
ggccgacctg 41 0.27% Ribosomal protein RPL11 2.00E-56/5.00E-73 S. pombe Z69240
catctattcc 40 0.26% NO HITS
cacgttcacg 40 0.26% Thioredoxin peroxidase 2.00E-39/2.00E-64 S. pombe AL031798
gcattggcgt 39 0.25% ER chaperone BiP 0.0/5.00E-20 Aspergillus oryzae AB030231
actatcgcct 38 0.25% Ubiquitin conjugating enzyme 8.00E-45/2.00E-75 Glomerella cingulata AF030296
gctcgcgacg 36 0.23% 60S ribosomal protein RPL2 2.00E-72 D. melanogaster AF098520
tccttccgag 36 0.23% Glyceraldehyde-3-phosphate dehydrogenase 1.00E-112/0.0 C. neoformans AF106950
cctgttctcg 36 0.23% NO HIT
tctgtcgagg 35 0.23% 40S ribosomal protein RPS12 6.00E-36/7.00E-42 S. pombe AL031154
cattcgttca 35 0.23% NO HIT
tagcctttcg 34 0.22% NO HIT
atgggctccc 34 0.22% ATP synthase—γ-chain 6.00E-44/9.00E-66 S. pombe AL031856
gctcctctta 33 0.21% ATP synthase—α-chain 2.00E-58/9.00E-46 S. pombe M57955
acatcgatct 32 0.21% 60S ribosomal protein RPL31 3E-25 Cyanophora paradoxa AJ005204
gcagatcgat 32 0.21% 60S ribosomal protein RPL39 3.00E-09/1.00E-13 K. marxianus S53434
gatgcttttt 30 0.20% 60S ribosomal protein RPL19 9.00E-31 S. pombe AB010048
ggttacgctg 30 0.20% Mitochondrial malate dehydrogenase 1.00E-115 S. cerevisiae J02841
cggtgcctgc 30 0.20% 60S ribosomal protein RPL15 3.00E-47/4.00E-88 Quercus suber AJ001346
aaatggtttg 29 0.19% NO HIT
gctaacgctg 29 0.19% Cyclophilin A 5.00E-76/2.00E-91 C. neoformans AF333996
aaccgcacca 29 0.19% Peripheral benzodiazepine receptor 6.00E-16 Homo sapiensJE0149
agtcctcttc 28 0.18% 60S ribosomal protein RPP2 1E-15 Alternaria alternata U87806
cagcagttta 28 0.18% NO HITS
cacggcgcat 27 0.18% 60S ribosomal protein RPL41 2E-39 C. neoformans AF118148
tagccgggaa 27 0.18% NO HITS
catagttggt 27 0.18% Heat shock protein 70 family 0 Malassezia sympodialis AJ428052

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  1. Genome Res. 12: 1386-1400

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