Figure 3.
RNA blot analysis of two representative temperature-regulated genes in strain B3501. The RNA was isolated from cells grown at 25°C or 37°C. (A) Hybridization with a polymerase chain reaction (PCR) amplicon from a gene for a heat shock protein 70 (tag, CATAGTTGGT) with a higher transcript level at 37°C (HS). (B) Hybridization with a PCR amplicon from a gene for a high-affinity monosaccharide transporter (tag, GGCTTGACCA) with a higher transcript level at 25°C (HXT). (C) Ribosomal RNA (18S and 28S) bands as a loading control.
