Voluntary Ethanol Consumption by Mice: Genome-Wide Analysis of Quantitative Trait Loci and Their Interactions in a C57BL/6ByJ × 129P3/J F2 Intercross

Table 3.

Epistatic Interactions Detected by the EpistatProgram and Confirmed by ANOVA

Marker 1 Marker 2 Epistat ANOVA
interaction LLR interaction P-value marker 1 P-value marker 2 P-value R2 d.f. interaction F-value interaction P-value
3% Ethanol intake
D2Mit61 D17Mit51 8.2 0.0067 0.17 0.004 0.068 1, 25 7.9 0.0094
D3Mit86 D13Mit147 12.8 0.0002 0.02 0.65 0.13 1, 31 13.2 0.00099
D6Mit55 D13Mit35 9.3 0.0042 0.30 0.95 0.029 1, 35 9.7 0.0036
D7Mit7 D13Mit44 9.7 0.0031 0.009 0.71 0.13 1, 43 12.2 0.0011
D10Mit194 D14Mit52 21.6 0.0005 0.76 0.21 0.0034 1, 27 11.0 0.0026
D13Mit97 DXMit69 11.4 0.0005 0.90 0.40 0.00027 1, 46 11.9 0.0012
D14Mit82 D18Mit33 12.4 0.0009 0.53 0.66 0.014 1, 27 17.0 0.00032
3% Ethanol preference
D1Mit19 D16Mit6 8.1 0.0101 0.51 0.48 0.018 1, 22 9.8 0.0049
D1Mit48 D12Mit194 9.1 0.0026 0.39 0.89 0.022 1, 31 9.0 0.0052
D1Mit37 D15Mit96 9.4 0.0011 0.08 0.17 0.10 1, 27 14.1 0.00083
D3Mit25 D6Mit36 10.6 0.0008 0.69 0.41 0.0041 1, 35 10.4 0.0028
D3Mit10 D17Mit6 8.7 0.0045 0.37 0.91 0.027 1, 29 9.0 0.0054
D4Mit4 D8Mit271 11.2 0.0005 0.39 0.01 0.021 1, 33 8.4 0.0067
D4Mit58 D19Mit35 8.7 0.0020 0.03 0.39 0.11 1, 33 10.8 0.0024
D6Mit36 D13Mit35 8.6 0.0021 0.46 0.42 0.017 1, 30 15.1 0.00053
D8Mit271 D9Mit218 20.3 0.0003 0.22 0.63 0.043 1, 32 13.1 0.00099
D9Mit306 D19Mit10 12.1 0.0032 0.43 0.96 0.017 1, 35 10.5 0.0026
D11Mit199 D16Mit6 10.9 0.0044 0.50 0.53 0.014 1, 31 10.7 0.0026
10% Ethanol intake
D2Mit61 D18Mit144 8.6 0.0007 0.18 0.25 0.052 1, 32 15.9 0.00037
D6Mit36 D13Mit35 29.2 <0.0001 0.42 0.03 0.020 1, 30 15.3 0.00049
D7Mit15 D13Mit44 9.1 0.0012 0.94 0.79 0.00019 1, 27 15.6 0.00051
D8Mit56 D16Mit71 9.1 0.0045 0.41 0.02 0.018 1, 35 7.8 0.0080
D11Mit184 D19Mit10 8.5 0.0025 0.25 0.92 0.032 1, 38 8.9 0.0050
10% Ethanol preference
D1Mit48 D12Mit20 16.8 0.0010 0.23 0.38 0.041 1, 34 12.9 0.0010
D1Mit37 D15Mit96 17.3 0.0005 0.03 0.79 0.15 1, 27 19.1 0.00016
D2Mit61 D18Mit144 9.1 0.0013 0.22 0.06 0.044 1, 32 16.4 0.00031
D3Mit89 DXMit69 9.2 0.0030 0.007 0.23 0.14 1, 43 12.8 0.00089
D5Mit214 D8Mit190 41.7 0.0005 0.89 0.33 0.00053 1, 36 8.1 0.0071
D6Mit36 D13Mit35 18.7 0.0082 0.50 0.01 0.014 1, 30 21.4 0.000068
D6Mit201 D11Mit199 16.3 0.0057 0.97 0.45 0.00005 1, 35 7.7 0.0088
D10Mit162 D13Mit34 16.8 0.0002 0.89 0.17 0.00050 1, 42 9.0 0.0045
D12Mit46 D15Mit193 10.0 0.0063 0.73 0.78 0.0032 1, 36 7.8 0.0085

  • LLR, log likelihood ratio. R2 represents portion of phenotypical variation controlled by the interacting loci.

  • ↵Single marker showing significant effect on a phenotype in theEpistat analysis (P < 0.05), but with no linkage to the trait in the Mapmaker analysis (LOD < 0.8).

  • ↵Single marker showing significant effect on a phenotype in theEpistat analysis (P < 0.05) and located in a region of significant or suggestive linkage. LOD scores are: 2.1 (D17Mit51 and 3% ethanol intake), 1.0 (D13Mit35and 10% ethanol intake), 1.4 (D13Mit35 and 10% ethanol preference), and 1.7 (D3Mit89 and 10% ethanol preference).

This Article

  1. Genome Res. 12: 1257-1268

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