NORFs Identified by MudPIT Proteomic Analyses
| NORF ID | Proposed ORF designation | Chromosomal location | Size (AA) | CAI | Peptide identified | Transcripts per cell |
| NAL010C | YAL063C-A | Chr I: 22400-22688 | 96 | 0.17 | R.YRNKEKGKFISLCK.N | 1.8 |
| NBR028W | YBR126C-A | Chr II: 490808-491014 | 68 | 0.16 | R.LHQLDGIPHA.- | N.D. |
| NCR024W | YCR095W-A | Chr III: 289632-289790 | 52 | 0.08 | H.TKVNKKSSMHAFLCKIYK.R | 0.7 |
| NDR129W | YDR320W-B | Chr IV: 1108476-1108613 | 45 | 0.13 | L.NSLLFLPICFCLLQLKATCAVR.V | 2.0 |
| NDR156C | YDR379C-A | Chr IV: 1233506-1233267 | 79 | 0.16 | K. DFTTIEHLLRVGNK.K | 1.4 |
| K.ENQVNFVNYIHEEFGK.Y | ||||||
| NGR097C | YGR169C-C | Chr VII: 836660-836382 | 92 | 0.13 | K.ERDALLTAEEEQLGK.G | 1.0 |
| K.ERDALLTAEEEQLGKGGK.G | ||||||
| K.QRAQMEQLEAEEASK.W | ||||||
| X.QRAQMEQLEAEEASKWEQGSRK.E | ||||||
| NHL007C | YHL048C-A | Chr VIII: 5796-5662 | 44 | 0.08 | G.RARMGGLIVKHRFN.H | 1.2 |
| NHR007W | YHR032W-A | Chr VIII: 175186-175365 | 59 | 0.06 | G.NFKGFAMWHATGKH.H | 0.7 |
| NIL001W | YIL002W-A | Chr IX: 350298-350507 | 69 | 0.17 | K.DILDVLNLLK.G | 1.5 |
| NIR003C | YIR018C-A | Chr IX: 385698-385561 | 45 | 0.10 | K.RYLEIMSTASAQ.F | 1.0 |
| NIR008W | YIR021W-A | Chr IX: 398511-398723 | 70 | 0.11 | K.SDFKKHSKE.I | 0.7 |
| NLR127C | YLR361C-A | Chr XII: 849678-849382 | 98 | 0.12 | R.TGGHRPQISDEEVSK.R | 0.8 |
| NMR066W | YMR247W-A | Chr XIII: 769282-769425 | 47 | 0.10 | S.AKLLSGIMALLFNGKSLLRP.I | 0.5 |
| NNL014W | YNL042W-B | Chr XIV: 547109-547366 | 85 | 0.11 | V.RVATYICQKNESR.F | 0.5 |
| NNL029W | YN067W-B | Chr XIV: 499414-499554 | 46 | 0.11 | L.MWCTGVVSKTALLTGNFFFS.S | 0.4 |
| NNL042C | YNL146C-A | Chr XIV: 351577-351383 | 64 | 0.13 | S.AYYVSQVLRICKEMPYR.D | 0.2 |
| NNL058W | YNL277W-A | Chr XIV: 116677-116865 | 62 | 0.05 | M.CHILPPLR.S | −0.2 |
| NOL015W | YOL077W-A | Chr XV: 185437-185643 | 68 | 0.20 | L.GLLGLLVVPNPFK.S | 2.2 |
| NOL020W | YOL097W-A | Chr XV: 136219-136404 | 61 | 0.12 | Q.SMICSSEHENLTCK.Y | 0.2 |
| NOL049W | YOL155W-A | Chr XV: 27083-27217 | 44 | 0.08 | G.SFNKCVTGYSCRMAIHYY.V | 0.0 |
| NOR002C | YOR034C-A | Chr XV: 397667-397425 | 80 | 0.13 | R.IWVREKGRKCSFFFF.S | 0.8 |
| NPL013C | YPL119C-A | Chr XVI: 324286-324023 | 87 | 0.11 | R.NIFEIGGLLLQ.S | 0.5 |
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↵CAI values were calculated according to Sharp and Li (1987).
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↵The transcriptional expression level of each NORF in approximate copies of transcripts per cell in a log phase cell.
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↵Probes to NBR028W were not selected for inclusion on the S98 array.
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↵YOL077W-A was discovered by the biochemical purification of the F1F0-ATP synthase complex (Arnold et al. 1998).
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↵The ‘-’ indicates the C-terminus of the protein.
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↵The ‘.’ after an amino acid indicates the cleavage sites of the peptide. The sequence between the periods in each cell indicates the actual peptide identified by tandem mass spectrometry. (ND) there was no detectable expression in a log phase culture.
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MudPIT, multidimensional protein identification technology.
