Mass spectra for a peptide from the NORF NIL001W. A multidimensional protein identification technology (MudPIT) analysis of the soluble proteome of BJ5460 was performed and the results analyzed via SEQUEST (Eng et al. 1994) using a concatenated database containing ORFs and NORFs. In the MudPIT analyses, a collision-induced dissociation tandem mass spectrum for (M + 2H) 2+ ion of the peptide DILDVLNLLK at m/z 578.5 from the NORFNIL001W was detected and identified. An eight-ion band seven-ion y series are shown in red and blue, respectively, and the corresponding amino acid difference between each ion is shown. The SEQUEST result for the tandem mass spectrum shown had an Xcorr of 3.1276 and a ΔCn of 0.2292, indicating complete confidence in the SEQUEST result.
