Functional Cloning, Sorting, and Expression Profiling of Nucleic Acid-Binding Proteins

Table 1a.

List of Clones Obtained from the Primary Screen

Clone no. Gene name  UniGene no. No. of hits Motif 
Adeno Fetal
 1 PTB Hs. 172550 3 0 RRM
 2 HNRPDL Hs. 170311 1 7 RRM
 3 YB-1/dpbB Hs. 74497 19 8 CSD
 4 Nucleolin Hs. 79110 3 1 RRM
 5 Topoisomerase 3 Hs. 91175 3 0 Several
 6 CSDA/dbpA Hs. 198726 13 1 CSD
 7 ASF Hs. 73737 1 0 RRM
 8 SFRS5 Hs. 166975 1 1 RRM
 9 HNRPAB Hs. 81361 3 0 RRM
10 CPSF4 (30K) Hs. 6351 5 0 zf (CCCH)
11 PEG10 Hs. 137476 0 1 bZip and zf (CCHC)
12 CIRBP Hs. 119475 1 7 RRM
13 Novel 405_111 NA 0 1 RRM
14 ZNF9 Hs. 2110 0 1 zf (CCHC & NF-X1)
15 HNRPM Hs. 79024 2 4 RRM
16 SPRS4 Hs. 76122 1 0 RRM
17 Novel 346_711 NA 1 0 Unknown
18 RBMS2 Hs. 20938 0 1 RRM
19 EIF4H Hs. 180900 0 1 RRM
20 PABPC1 Hs. 172182 13 5 RRM & PABP
21 PABPC4 hs. 169900 10 1 bZip, RRM & PABP
22 HNRPA 1 Hs. 249495 4 14 RRM
23 HNRPD Hs. 303627 0 10 RRM
24 Novel 405_311 NA 0 1 R3H
25 Novel 412_111 NA 0 1 Coiled coil DBD
26 Novel 418_321 NA 0 1 RRM
27 MSSP-2 Hs. 241567 0 1 RRM
28 RBMS1 Hs. 241567 0 4 RRM
29 U2AF65 Hs. 7655 1 0 RRM
30 Novel 421_111 NA 0 1 RRM
  • The first column refers to the clone number, second column to the gene identity and third column to the corresponding UniGene number. Novel genes are represented in bold and NA refers to not applicable. The fourth column provides information on the corresponding number of hits of each gene from the two library screens (Adeno library made from adeno-carcinoma cell line and Fetal library made from fetal-lung tissue). The fifth column provides information on the predicted nucleic acid-binding motif present in the protein encoded by the corresponding gene (as determined by searching the PFAM database).

This Article

  1. Genome Res. 12: 1175-1184

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