Validation of DNA Sequences Using Mass Spectrometry Coupled with Nucleoside Mass Tagging

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Figure 2.
Figure 2.

Negative-ion matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) spectra of the unlabeled (UL) and 99% 13C/15N-dCTP-labeled (L) PCR products amplified from genomic DNA with unlabeled dNTPs (A), 99%13C/15N-labeled dCTP (B), and equal amounts of unlabeled dNTPs and 99% 13C/15N-labeled dCTP (C). “+Na+” indicates sodium adducts. All spectra were obtained using an accelerating voltage of 20 kV, a grid voltage of 95% with 128 average scans, and an extraction delay time of 600 ns.

This Article

  1. Genome Res. 12: 1135-1141

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