Multiple alignment of full-length cDNAs belonging to the same group and having the same functional annotation using the ClustalXprogram (Thompson et al. 1997). (A) This group contains four cDNAs of the histone 4 protein (AK016310, AK007642, AK011560, andAK010085). These sequences have a homologous region and a variable region, so that these sequences were placed together in grouping but separated into distinct clusters. We compared these sequences with mouse draft genome sequences. As a result, these sequences matched all distinct loci on the mouse draft genome, indicating that they were derived from distinct genes. With regard to grouping, one clone is selected as the representative, but all clones are selected as representatives after clustering. (B) This group contains four cDNAs of the acidic ribosomal phosphoprotein PO (AK002315, AK009767,AK010267, and AK012606). The 3′ ends of the upper two cDNA sequences differ in length by ≤20 bp; therefore, these sequences are regarded as the same cDNAs after clustering. These sequences matched the same locus on the mouse genome. However, the lengths of the 3′ ends of the other two cDNA sequences differ by >20 bp, so that these sequences separate into distinct clusters. These sequences may match another locus on the mouse genome. With regard to grouping, one clone is selected as the representative, but three clones are selected as representatives after clustering.
