Figure 5.
Identification of a new functional motif using AlignACE. (A) Sequence logo (Schneider and Stephens 1990) showing the motif A core as identified by AlignACE. (B) Sequence logo for the POU/homeodomain TF Oct-1 from TRANSFAC (Wingender et al. 2001; accession #M00342). (C) Alignments of motif A from the Eve MHE and Hbr DME with the corresponding sequences fromD. virilis. (D) Wild-type and (E) motif A site mutated Eve MHE reporter gene expression (red) along with endogenous Eve expression (green). The motif A mutation leads to an expansion of reporter gene expression along the anteroposterior axis (arrows).
