An Integrated Computational and Laboratory Approach for Selective Amplification of mRNAs Containing the Adenylate Uridylate-Rich Element Consensus Sequence

Table 3.

Summary of Identity of ARE-Expressed Sequences

Total: 30 distinct clones
cDNA/mRNA with known function (7)
Interleukin-1β (4 AREs)
c-fos (3 AREs)
Hypoxia-induced factor α (3 AREs)
Plasminogen activator inhibitor protein (2 AREs)
Diacylglycerol kinase delta (1 ARE)
Amyloid beta (A4) precursor (1 ARE)
Small inducible cytokine A4 (1 ARE)
 Matched EST hits (9)
 Matched EST + cDNA (16)
 No significant matches (14)
  • ARE-PCR products generated from random cloning of pUC19 ARE mini-library or excised from ARE-cDNA differential display gel were sequenced. Sequence identities were generated using BLASTsearch of GenBank and hEST databases. ARE refers to the 13-bp computationally derived ARE motif consensus, that is, WW WTATTTATWWWBakheet et al. 2001).

  • The accession numbers BG604230 to BG604251 were obtained from dbEST for the sequence data in this article.

  • ARE, adenylate uridylate-rich element; EST, expressed sequence tag; PCR, polymerase chain reaction.

This Article

  1. Genome Res. 12: 985-995

Preprint Server