Localization of Functionally Characterized Binding Sites to Conserved Blocks
| Location | Ref | Element sequence | NFAT (%ID) | AP-1 (%ID) | ||
| IL-2 | E1 | 280 | (15) | AGGAAAATTTGTTTCATA | 90.48 | 90.48 |
| E2 | 160 | (15) | AGAAATTCCAGAGAGTCA | 90.48 | 95.24 | |
| E3 | 135 | (15) | AGGAAAAACAAAGGTAAT | 95.24 | 80.95 | |
| E4 | 90 | (15) | TTGAAAATATGTGTAATA | 95.25 | 100 | |
| E5 | 45 | (15) | TGGAAAAAT | 95.25 | n/a | |
| IL-3 | E6 | 306 | (11) | TGAGCTGAGTCAGGCTTCCCCTT | 76.2 | 71.43 |
| IL-4 | P0 | −106 | (1, 2) | GTAAACTCATTTTCCCTTGGTTTC | 95.25 | 100 |
| P1 | −121 | (1, 2) | GTAATAAAATTTTCCAATGTAAAC | 95.25 | 100 | |
| P2 | −124 | (1, 2) | ACAGGTAAATTTTCCTGTGAAATC | 95.25 | 100 | |
| P3 | −238 | (1, 2, 3) | GGTGTTTCATTTTCCAATTTGTCT | 95.25 | 95.24 | |
| P4 | −287 | (1, 2) | TATGGTGTAATTTCCTATGCTTGA | 100 | 100 | |
| P5 | −406 | (16) | GCAGTCCTCCTGGGGAAAGATAGAGTAATATCA | 95.25 | 95.24 | |
| IL-5 | E7 | 163 | (4, 6, 7) | GCATTGGAAACATTTAGTTTCACGAT | 80.95 | 80.95 |
| E8 | 104 | (4, 5) | GAAATTATTCATTTCCTCAAAG | 90.48 | 95.24 | |
| IL-13 | E9 | 135 | (12) | CTGGATTTTCCA | 85.71 | n/a |
| E10 | 154 | (13) | CATGAGAAATCAAATCTTTCCTTTA | 90.48 | 80.95 | |
| GMCSF | E11 | −67 | (9, 10) | CACCATTAATCATTTCCTCTG | 80.95 | 100 |
| E12 | −101 | (14) | AGGAGATTCCACAGTTCAGGTAGTTCCCCCGCCTCC | 95.1 | 90.48 | |
| E13 | −163 | (13) | CCTAGGGAAAAGGCTCACCGT | 80.95 | 90.48 | |
| E14 | −2.8kb | (8) | GCCCTGATGTCATCTTTCCATGA | 90.48 | 90.48 | |
| E15 | −2.9kb | (8) | CCATCGGAGCCCCTGAGTCAGCAT | 85.71 | 85.71 | |
| GATA-3 (%ID) | Distance (bp) | |||||
| IL-4 | E16 | −87 | (17) | ATTACACCAGATTGTCAGTTA- | 95.25 | 20 |
| TTCTGGGCCAATCAGCACC | 100 | |||||
| IL-5 | E17 | −75 | (18) | CTCTATCTGATTGTTA | 95.24 | 5 |
| 95.24 |
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This table represents a collection of functionally characterized NFAT, AP-1, and GATA-3 binding sites. Element position is indicated in reference to the beginning of the 5′UTR of the gene. AP-1 and NFAT binding sites were independently identified using TRANSFAC (0.75/0.8) and compared with the published sequences. Distance between adjacent GATA-3 sites was measured between beginning positions of the cores. Base pairs (bp).
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↵TFBS below default parameters. Percent identity (%ID) for each aligned TFBS (21 bp) was determined by rVISTA (n/a) not applicable. (1) Szabo et al. (1993); (2) Takemoto et al. (1997); (3) Li-Weber et al. (1994); (4) Lee et al. (1995); (5) Thomas et al. (1999); (6) Prieschl et al. (1995); (7) Stranick et al. (1997); (8)Cockerill et al. (1995); (9) Jenkins et al. (1995); (10) Masuda et al. (1993); (11) Gottshalk et al. (1993); (12) De Boer et al. (1999); (13)Kel et al. (1999); (14) Cakouros et al. (2001); (15) Rooney et al. (1995); (16) Burke et al. (2000); (17) Zheng and Flavell (1997); (18)Lee et al. (1998).
