
FISH analysis of CTG4-1. Three BAC clones, OSJNBa0068L06 (red signal), OSJNBb0085F13 (green signal), and OSJNBa0042N22 (pink signal), within CTG4-1 were chosen for FISH analysis to confirm the contig localization and orientation. RFLP markers R2502 (3.1 cM) and R2373 (0 cM) matched OSJNBa0068L06 and OSJNBb0004G23, respectively. Because OSJNBb0004G23 and OSJNBb0085F13 are overlapped, the order of the two markers was confirmed on the physical map by FISH analysis. Therefore, their genetic order is reversed on the physical map.











