Figure 3.
BAC-FISH mapping of a pachytene chromosome 4. FISH of OSJNBb0085F13 (red signal) and OSJNBb0020J19 (green signal) revealed that they are near the telomeres of the short and long arms, respectively. The centromere of the chromosome 4 was detected by a rice centromere-specific satellite DNA pRCS2 as described previously (Dong et al. 1998) and indicated in yellow. The short arm and the pericentromeric region of the long arm are deeply stained by DAPI and therefore represent the heterochromatin-rich regions, which occupied almost one-third of the chromosome when visually measured.
