Number of Contigs Annotated with Selected GO Nodes and Their Children Nodes in the Three GO Categories, and Chi-Square Test Results
| GO term | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | X | Y | Contig | Chi | P value |
| Physiological process | |||||||||||||||||||||||||||
| Spermatogenesis | 4 | 4 | 2 | 1 | 1 | 7 | 1 | 9 | 3 | 3 | 2 | 3 | 1 | 1 | 1 | 3 | 1 | 0 | 1 | 7 | 1 | 2 | 8 | 11 | 77 | 484 | 0.00E-00 |
| Homophilic cell adhesion | 2 | 0 | 1 | 5 | 24 | 0 | 1 | 1 | 0 | 2 | 3 | 0 | 4 | 1 | 0 | 11 | 0 | 9 | 0 | 3 | 0 | 1 | 3 | 1 | 72 | 235 | L04E-37 |
| Olfaction | 4 | 0 | 0 | 0 | 0 | 12 | 5 | 1 | 4 | 0 | 25 | 1 | 1 | 1 | 0 | 2 | 7 | 0 | 5 | 0 | 0 | 0 | 0 | 0 | 68 | 165 | 5-45E-24 |
| DNA-dependent DNA replication | 8 | 5 | 4 | 6 | 2 | 8 | 23 | 1 | 1 | 4 | 1 | 4 | 4 | 2 | 0 | 5 | 8 | 5 | 2 | 5 | 0 | 6 | 1 | 0 | 105 | 103 | L75E-12 |
| Transcription regulation | 143 | 100 | 105 | 49 | 67 | 93 | 93 | 71 | 87 | 52 | 106 | 102 | 41 | 50 | 55 | 76 | 85 | 39 | 171 | 67 | 11 | 34 | 68 | 6 | 1771 | 97 | 2.10E-11 |
| Immune response | 96 | 52 | 43 | 31 | 29 | 44 | 24 | 16 | 20 | 21 | 45 | 29 | 4 | 21 | 22 | 13 | 30 | 8 | 65 | 15 | 8 | 24 | 15 | 0 | 675 | 73 | 1.84E-07 |
| Ribosome biogenesis | 7 | 5 | 4 | 2 | 8 | 3 | 4 | 2 | 4 | 0 | 3 | 5 | 1 | 2 | 3 | 3 | 3 | 1 | 4 | 1 | 0 | 0 | 2 | 0 | 67 | 14 | 0.88 |
| Cell shape and cell size control | 11 | 10 | 5 | 1 | 7 | 6 | 7 | 2 | 7 | 3 | 10 | 8 | 2 | 3 | 5 | 3 | 8 | 2 | 6 | 3 | 1 | 4 | 1 | 0 | 115 | 14 | 0.90 |
| Exocytosis | 20 | 8 | 13 | 7 | 10 | 8 | 13 | 5 | 5 | 8 | 10 | 14 | 2 | 7 | 7 | 5 | 9 | 3 | 7 | 6 | 0 | 5 | 3 | 0 | 175 | 13 | 0.90 |
| Central nervous system development | 9 | 8 | 4 | 5 | 4 | 3 | 5 | 3 | 4 | 2 | 7 | 8 | 2 | 2 | 4 | 5 | 7 | 0 | 7 | 1 | 2 | 1 | 3 | 0 | 96 | 12 | 0.94 |
| Molecular function | |||||||||||||||||||||||||||
| Calcium-dependent cell adhesion molecule | 1 | 0 | 1 | 4 | 23 | 0 | 1 | 1 | 0 | 2 | 2 | 0 | 4 | 1 | 0 | 10 | 0 | 9 | 0 | 3 | 0 | 1 | 2 | 1 | 66 | 238 | 2.59E-38 |
| Olfactory receptor | 6 | 1 | 1 | 0 | 1 | 13 | 5 | 3 | 9 | 0 | 31 | 1 | 1 | 1 | 0 | 3 | 8 | 0 | 9 | 0 | 0 | 0 | 0 | 0 | 93 | 181 | 4338-27 |
| Tumor antigen | 14 | 4 | 0 | 1 | 1 | 2 | 4 | 2 | 3 | 2 | 3 | 5 | 5 | 2 | 0 | 0 | 2 | 3 | 5 | 1 | 1 | 2 | 23 | 0 | 85 | 161 | 3.20B-23 |
| G-protein coupled receptor | 94 | 50 | 59 | 30 | 50 | 84 | 41 | 23 | 30 | 28 | 76 | 33 | 24 | 30 | 9 | 16 | 56 | 11 | 85 | 23 | 2 | 18 | 59 | 1 | 932 | 130 | 2.43E-17 |
| Defense/humanity protein | 75 | 45 | 33 | 16 | 21 | 46 | 20 | 18 | 15 | 9 | 30 | 14 | 6 | 17 | 19 | 7 | 11 | 4 | 55 | 14 | 6 | 22 | 10 | 0 | 513 | 106 | 4.10E-13 |
| Transcription factor | 116 | 78 | 80 | 37 | 58 | 78 | 77 | 55 | 74 | 52 | 95 | 79 | 24 | 41 | 51 | 59 | 60 | 33 | 149 | 51 | 9 | 24 | 59 | 4 | 1443 | 100 | 5.04E-12 |
| Glycopeptide hormone | 6 | 6 | 2 | 0 | 3 | 4 | 3 | 1 | 1 | 1 | 1 | 5 | 2 | 2 | 1 | 1 | 3 | 1 | 5 | 1 | 1 | 1 | 3 | 0 | 54 | 13 | 0.91 |
| Metallopeptidase | 14 | 8 | 11 | 6 | 7 | 6 | 8 | 8 | 4 | 10 | 13 | 13 | 3 | 3 | 5 | 6 | 7 | 3 | 7 | 6 | 2 | 2 | 4 | 0 | 156 | 13 | 0.92 |
| Serotonin receptor | 11 | 9 | 4 | 3 | 7 | 4 | 7 | 4 | 3 | 5 | 11 | 6 | 2 | 1 | 3 | 6 | 6 | 0 | 6 | 4 | 0 | 3 | 3 | 0 | 108 | 13 | 0.92 |
| Protein serine/threonine kinase | 56 | 43 | 32 | 23 | 24 | 29 | 23 | 16 | 23 | 21 | 23 | 31 | 8 | 15 | 18 | 18 | 29 | 5 | 27 | 11 | 5 | 7 | 20 | 1 | 508 | 11 | 0.97 |
| Cellular component | |||||||||||||||||||||||||||
| Secretory vesicle | 5 | 17 | 5 | 1 | 1 | 2 | 3 | 2 | 2 | 5 | 9 | 5 | 1 | 8 | 6 | 4 | 8 | 1 | 1 | 2 | 1 | 12 | 3 | 0 | 104 | 88 | 6.09E-10 |
| Intermediate filament | 6 | 5 | 3 | 2 | 3 | 9 | 2 | 2 | 1 | 2 | 2 | 12 | 0 | 0 | 1 | 3 | 18 | 0 | 0 | 3 | 1 | 2 | 3 | 0 | 80 | 86 | 1.30E-09 |
| 26S proteasome | 11 | 7 | 5 | 1 | 0 | 2 | 3 | 1 | 2 | 3 | 5 | 2 | 1 | 7 | 3 | 5 | 20 | 2 | 2 | 1 | 2 | 1 | 1 | 0 | 87 | 85 | 1.72E-09 |
| Nuclear membrane lumen | 173 | 91 | 87 | 50 | 72 | 79 | 76 | 57 | 64 | 83 | 83 | 97 | 23 | 39 | 63 | 70 | 60 | 48 | 78 | 49 | 12 | 33 | 51 | 22 | 1560 | 82 | 6.79E-09 |
| Collagen | 9 | 11 | 7 | 0 | 1 | 5 | 2 | 5 | 2 | 1 | 5 | 1 | 1 | 7 | 0 | 1 | 2 | 0 | 2 | 2 | 4 | 8 | 3 | 0 | 79 | 78 | 3.12E-08 |
| Respiratory chain complex I | 6 | 4 | 4 | 1 | 5 | 2 | 2 | 1 | 3 | 1 | 4 | 1 | 0 | 2 | 3 | 3 | 4 | 0 | 3 | 0 | 1 | 2 | 2 | 0 | 54 | 12 | 0.94 |
| NADH dehydrogenase (abiquinone) | 6 | 4 | 4 | 1 | 5 | 2 | 2 | 1 | 3 | 1 | 4 | 1 | 0 | 2 | 3 | 3 | 4 | 0 | 3 | 0 | 1 | 2 | 2 | 0 | 54 | 12 | 0.94 |
| Mitochondrial inner membrane | 36 | 31 | 35 | 15 | 25 | 24 | 16 | 17 | 16 | 15 | 28 | 23 | 11 | 13 | 13 | 22 | 23 | 8 | 21 | 12 | 7 | 10 | 14 | 2 | 437 | 12 | 0.95 |
| Small ribosomal subunit | 9 | 6 | 4 | 3 | 4 | 3 | 6 | 2 | 3 | 1 | 4 | 3 | 0 | 3 | 1 | 6 | 4 | 1 | 3 | 3 | 1 | 2 | 2 | 0 | 74 | 11 | 0.96 |
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Numbers or x, y in the column headings indicate the chromosome. The results indicate that contigs annotated with some GO nodes or their children nodes such as spermatogenesis and homophilic cell adhesion under “Physiological Process,” or tumor antigen and defense/immunity protein under “Molecular function,” or secretory vesicle and intermediate filament under “Cellular component,” among many others, are distributed unevenly across different chromosomes.
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↵Total number of contigs the annotated with the GO node listed under GO term column or its children nodes.
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↵CHi-square test score.
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↵Significance level from the chi-square test.
