FISH analyses for evolutionary studies. (A) Metaphase and interphase cells of chimpanzee, gorilla, orangutan, gibbon, baboon, and rhesus after hybridization with SMS-REP bacterial artificial chromosome (BAC) RP11–158M20. (B) The interphase nuclei of New World squirrel monkey after cohybridization of differentially labeled SMS-REP-specific BAC (RP11–158M20) and a P1 artificial chromosome clone that directly flanks SMS-REP (RP1–178F10). Note the single yellow foci, indicating the <100-kb physical vicinity of the clones. Similar results were obtained using five other clones directly flanking individual SMS-REPs, showing the presence of three copies of SMS-REPs.
