Identification of a Novel cis-Regulatory Element Involved in the Heat Shock Response in Caenorhabditis elegans Using Microarray Gene Expression and Computational Methods

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Figure 5.
Figure 5.

Effect of HSAS element on reporter transgene expression. First generation (F1) transgenic animals containing a GFP reporter construct with either both HSE2 and HSAS mutated (HSE1 only) or HSE2 mutated (HSE1 + HSAS) were heat shocked at 35°C, returned to 20°C for 18 hr, then imaged for GFP expression. This digitally merged differential interference contrast/epifluorescence image shows the requirement for the HSAS element for strong transgene expression when the HSE is mutated. Size bar = 50 μm.

This Article

  1. Genome Res. 12: 701-712

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