Summary of mutated promoter construct experiments. (A) Thehsp-16–2/−41 promoter sequence in pPD122.18. Candidate promoter elements identified in bold font; sequences above the promoter elements indicate sequences introduced in mutated derivatives. TATA boxes underlined, transcriptional starts (Candido et al. 1989) indicated with grey nucleotides. Nucleotides in outlined font represent conserved site also found in F44E5.4/5 promoter region (see Fig. 3D). (B) Schematic of promoter element mutants and corresponding expression in transgenic animals. Transgenic F1 roller animals were scored as “strong GFP” (green fluorescence protein) if >50 GFP+ nuclei were observed. The two non-GFP animals observed in the pPD122.18 and H1 mutated construct injections likely result from rare events when the reporter construct was not incorporated in the Rol marker-containing array. Transgenic animals were scored as “any GFP” if at least one GFP positive nucleus could be observed. Footnotes: a, GFP+ animals from these injections contained an average of 5.5 (predominantly neuronal) GFP positive nuclei; b, one GFP positive animal was observed with 10 GFP+ head neurons; c, one head neuron in one animal was GFP positive.
