Table 11.
Homology Size (In Nucleotides) vs. Sensitivity for Four Search Criteria as Applied to Mouse/Human Comparisons Assuming 86% Nucleotide Identity and 89% Amino Acid Identity
| 20 | 30 | 40 | 50 | 60 | 70 | 80 | 90 | 100 | 110 | 120 | 130 | |
| A | 0.625 | 0.805 | 0.881 | 0.927 | 0.962 | 0.977 | 0.986 | 0.993 | 0.995 | 0.997 | 0.999 | 0.999 |
| B | 0.000 | 0.394 | 0.687 | 0.851 | 0.932 | 0.932 | 0.970 | 0.987 | 0.995 | 0.998 | 0.999 | 0.999 |
| C | 0.483 | 0.733 | 0.862 | 0.929 | 0.963 | 0.965 | 0.981 | 0.990 | 0.995 | 0.997 | 0.999 | 0.999 |
| D | 0.000 | 0.783 | 0.783 | 0.953 | 0.953 | 0.953 | 0.990 | 0.990 | 0.998 | 0.998 | 1.000 | 1.000 |
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The median size of a human exon is 120 bases (International Human Genome Sequencing Consortium 2001). A, perfect 5 base amino acid match; B, two perfect 4-base amino acid matches within 100 amino acids and on diagonal; C, near-perfect 12 nucleotide match; D, near-perfect 8 amino acid match.
