The Genomic Regions of PVA, Their Sizes, and the Numbers of Insertions Detected in Them
| Region | Size (nt) | No. of insertions | |||
| Total | Essential sites | Nonessential sites | |||
| 5′NTR | 161 | 22 | 20 | 2 | |
| P1 | 891 | 90 | 44 | 46 | |
| HC-Pro | 1374 | 152 | 60 | 92 | |
| P3 | 1041 | 135 | 33 | 102 | |
| 6K1 | 156 | 13 | 4 | 9 | |
| CI | 1905 | 218 | 60 | 158 | |
| 6K2 | 159 | 20 | 3 | 17 | |
| VPg | 567 | 63 | 20 | 43 | |
| NIa-Pro | 729 | 91 | 11 | 80 | |
| NIb | 1548 | 188 | 39 | 149 | |
| CP | 807 | 102 | 31 | 71 | |
| 3′NTR | 227 | 31 | 4 | 27 | |
| Total | 9565 | 1125 | 329 | 796 | |
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5′-NTR and 3′NTR: 5′- and 3′-nontranslated regions, respectively; P1, the first protein; HC-Pro, helper component-proteinase; P3, the third protein; 6K1 and 6K2, 6 kDa proteins 1 and 2; CI, cylindrical inclusion protein; VPg, viral genome-linked protein; NIa-Pro, proteinase; NIb, replicase; CP, coat protein.
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↵Essential sites are those in which insertions prevented virus propagation and/or production of mature virions in protoplasts. Distribution of insertions within each of the genomic regions is shown in Fig.7.
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↵Size in nucleotides (nt). For positions in the genome, see Fig.7.
